Hotspot

psbT-psbN

LSC · 75730-75815 · 86 bp

Back to Pachypleurum

Pi 0.031

MarkerSeek score 69.40

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 86
variable_sites: 4
pi: 0.031
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	GAGAACCGCCTAAAGTTCCA	TCGAGGAACATGGGGACTAG	50.0	55.0	57.5	58.2	138 bp	1.0	76.8
psbT-psbN_p2	GAGAACCGCCTAAAGTTCCA	TTCGAGGAACATGGGGACTA	50.0	50.0	57.5	57.8	139 bp	1.0	76.6
psbT-psbN_p3	GAGAACCGCCTAAAGTTCCA	GATCCCTTCGAGGAACATGG	50.0	55.0	57.5	57.8	145 bp	1.0	76.5
psbT-psbN_p4	GAGAACCGCCTAAAGTTCCA	GGGTACGCCTTATATACCGC	50.0	55.0	57.5	57.7	193 bp	1.0	75.6
psbT-psbN_p5	GAGAACCGCCTAAAGTTCCA	GTTTTACCGGGTACGCCTTA	50.0	50.0	57.5	57.6	201 bp	1.0	74.8