Hotspot

trnP-UGG-psaJ

LSC · 68047-68425 · 379 bp

Back to Pachypleurum

Pi 0.0088

MarkerSeek score 55.60

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 379
variable_sites: 5
pi: 0.0088
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	AAAACAAACGCGCTACCAAG	GGTGCCACGGAAAGATATGT	45.0	50.0	57.9	58.0	449 bp	1.0	79.7
trnP-UGG-psaJ_p2	CAAAACAAACGCGCTACCAA	GGTGCCACGGAAAGATATGT	45.0	50.0	58.2	58.0	450 bp	1.0	79.2
trnP-UGG-psaJ_p3	TTGTACCCAAAACAAACGCG	GGTGCCACGGAAAGATATGT	45.0	50.0	57.8	58.0	457 bp	1.0	79.0
trnP-UGG-psaJ_p4	AAAACAAACGCGCTACCAAG	GACCTGCTAAAGACCCGAAC	45.0	55.0	57.9	58.3	487 bp	1.0	77.7
trnP-UGG-psaJ_p5	TACCCAAAACAAACGCGCTA	GGTGCCACGGAAAGATATGT	45.0	50.0	58.4	58.0	454 bp	1.0	77.3