Hotspot

trnW-CCA-trnP-UGG

LSC · 67816-67972 · 157 bp

Back to Pachypleurum

Pi 0.0758

MarkerSeek score 83.30

Primer available yes

Label trnW-CCA-trnP-UGG

Feature Metadata

label_name: trnW-CCA-trnP-UGG
feature_type: igs
region: LSC
length_bp: 157
variable_sites: 16
pi: 0.0758
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 83.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnW-CCA-trnP-UGG_p1	GCGTTCTACCGAACTGAACT	CTTGGTAGCGCGTTTGTTTT	50.0	45.0	57.9	57.9	239-244 bp	1.0	85.6
trnW-CCA-trnP-UGG_p2	GCGTTCTACCGAACTGAACT	CGCGTTTGTTTTGGGTACAA	50.0	45.0	57.9	57.8	231-236 bp	1.0	85.2
trnW-CCA-trnP-UGG_p3	GCGTTCTACCGAACTGAACT	TTGGTAGCGCGTTTGTTTTG	50.0	45.0	57.9	58.2	238-243 bp	1.0	85.1
trnW-CCA-trnP-UGG_p4	ACCTACGACATCGGGTTTTG	CTTGGTAGCGCGTTTGTTTT	50.0	45.0	57.9	57.9	266-271 bp	1.0	84.6
trnW-CCA-trnP-UGG_p5	ACCTACGACATCGGGTTTTG	TTGGTAGCGCGTTTGTTTTG	50.0	45.0	57.9	58.2	265-270 bp	1.0	84.2