Hotspot

trnG-UCC-trnfM-CAU

LSC · 38246-38439 · 194 bp

Back to Pachypleurum

Pi 0.027

MarkerSeek score 67.20

Primer available yes

Label trnG-UCC-trnfM-CAU

Feature Metadata

label_name: trnG-UCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 194
variable_sites: 7
pi: 0.027
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnfM-CAU_p1	ATGCGGATATGGTCGAATGG	CTTGAGGTCACGGGTTCAAA	50.0	50.0	57.9	58.0	292-301 bp	1.0	83.2
trnG-UCC-trnfM-CAU_p2	TCTTTGCCAAGGAGAAGACG	CTTGAGGTCACGGGTTCAAA	50.0	50.0	57.8	58.0	263-272 bp	1.0	83.1
trnG-UCC-trnfM-CAU_p3	ATGCGGATATGGTCGAATGG	GGGTAGAGCAGTTTGGTAGC	50.0	55.0	57.9	58.0	328-337 bp	1.0	82.9
trnG-UCC-trnfM-CAU_p4	TCTTTGCCAAGGAGAAGACG	GGGTAGAGCAGTTTGGTAGC	50.0	55.0	57.8	58.0	299-308 bp	1.0	82.7
trnG-UCC-trnfM-CAU_p5	ATGCGGATATGGTCGAATGG	TGAGGTCACGGGTTCAAATC	50.0	50.0	57.9	57.8	290-299 bp	1.0	82.1