Hotspot

trnK-UUU-rps16

LSC · 5076-5784 · 709 bp

Back to Pachypleurum

Pi 0.0162

MarkerSeek score 63.10

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 709
variable_sites: 16
pi: 0.0162
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	TCCCTCGCTTCATCCAAATG	AGGAACTGTTCGGGATCTTT	50.0	45.0	58.0	56.5	814-833 bp	1.0	70.8
trnK-UUU-rps16_p2	CCGATGGTATGGACGAATCC	AGGAACTGTTCGGGATCTTT	55.0	45.0	57.9	56.5	831-850 bp	1.0	70.5
trnK-UUU-rps16_p3	CGATGGTATGGACGAATCCC	AGGAACTGTTCGGGATCTTT	55.0	45.0	57.9	56.5	830-849 bp	1.0	70.5
trnK-UUU-rps16_p4	AAGCCGAGTACTCTACCGTT	AGGAACTGTTCGGGATCTTT	50.0	45.0	58.2	56.5	769-788 bp	1.0	70.1
trnK-UUU-rps16_p5	AAAGCCGAGTACTCTACCGT	AGGAACTGTTCGGGATCTTT	50.0	45.0	58.2	56.5	770-789 bp	1.0	70.0