Hotspot

trnH-GUG-psbA

LSC · 992-1176 · 185 bp

Back to Pachypleurum

Pi 0.0757

MarkerSeek score 75.90

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 185
variable_sites: 21
pi: 0.0757
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	CCTCTAGACCTAGCTGCTGT	55.0	55.0	58.1	57.9	252-274 bp	1.0	84.8
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	266-288 bp	1.0	84.5
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	265-287 bp	1.0	84.5
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	267-289 bp	1.0	84.5
trnH-GUG-psbA_p5	GATCCACTTGGCTACATCCG	TCCTCTAGACCTAGCTGCTG	55.0	55.0	58.1	57.6	253-275 bp	1.0	82.5