Hotspot

ycf1

SSC · 119485-124656 · 5172 bp

Back to Pachyphytum

Pi 0.00955

MarkerSeek score 48.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5172
variable_sites: 47
pi: 0.00955
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 48.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGACCACTTTTGTTAATCCAGT	CCGTTCTATCTGAAACCTTGGT	36.4	45.5	55.6	58.1	263-266 bp	1.0	42.1
ycf1_p2	AGACCACTTTTGTTAATCCAGT	TCCGTTCTATCTGAAACCTTGG	36.4	45.5	55.6	57.8	264-267 bp	1.0	42.1
ycf1_p3	AGACCACTTTTGTTAATCCAGT	AGTGGTGTTCAACTATCCGA	36.4	45.0	55.6	56.2	338-341 bp	1.0	41.6
ycf1_p4	AGACCACTTTTGTTAATCCAGT	AGTGGTGTTCAACTATCCGAA	36.4	42.9	55.6	57.0	338-341 bp	1.0	41.6
ycf1_p5	AGACCACTTTTGTTAATCCAGTT	AGTGGTGTTCAACTATCCGA	34.8	45.0	56.3	56.2	338-341 bp	1.0	41.6