Hotspot

ccsA

SSC · 110431-111390 · 960 bp

Back to Pachyphytum

Pi 0.00521

MarkerSeek score 47.40

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 960
variable_sites: 5
pi: 0.00521
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	CTTGAGCATCTCGGTTCGAA	TGCATTACCGGGAATGAGTG	50.0	50.0	58.0	58.0	1662-1664 bp	1.0	80.7
ccsA_p2	CTTGAGCATCTCGGTTCGAA	GCGGGACCACGAGAATTATT	50.0	50.0	58.0	58.1	1451-1453 bp	1.0	80.7
ccsA_p3	TGGCGGCATACTCTCTTCTA	TGCATTACCGGGAATGAGTG	50.0	50.0	57.9	58.0	1636-1638 bp	1.0	80.2
ccsA_p4	CTTGAGCATCTCGGTTCGAA	GCTCTTGACGACTGCAACTA	50.0	50.0	58.0	57.9	1148-1149 bp	1.0	80.0
ccsA_p5	CTTGAGCATCTCGGTTCGAA	CTTCACTTGCATTACCGGGA	50.0	50.0	58.0	57.9	1671 bp	0.5	59.1