Hotspot

ccsA-ndhD

SSC · 111391-111641 · 251 bp

Back to Pachyphytum

Pi 0.044

MarkerSeek score 72.70

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 251
variable_sites: 11
pi: 0.044
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	GCAATTGTAGCTTCTATGGGC	TGGTATTTACCCGGATTTCGT	47.6	42.9	57.4	57.1	440-441 bp	1.0	53.8
ccsA-ndhD_p2	CCGCAATTGTAGCTTCTATGG	TGGTATTTACCCGGATTTCGT	47.6	42.9	57.1	57.1	442-443 bp	1.0	51.8
ccsA-ndhD_p3	CGCAATTGTAGCTTCTATGGG	TGGTATTTACCCGGATTTCGT	47.6	42.9	57.1	57.1	441-442 bp	1.0	51.8
ccsA-ndhD_p4	GCAATTGTAGCTTCTATGGGC	TGGTATTTACCCGGATTTCGTT	47.6	40.9	57.4	57.8	440-441 bp	1.0	51.4
ccsA-ndhD_p5	GCAATTGTAGCTTCTATGGGC	TTGGTATTTACCCGGATTTCGT	47.6	40.9	57.4	57.8	441-442 bp	1.0	51.4