Hotspot

trnL-UAG-ccsA

SSC · 110358-110430 · 73 bp

Back to Pachyphytum

Pi 0.0278
MarkerSeek score 69.80
Primer available yes
Label trnL-UAG-ccsA

Feature Metadata

label_name
trnL-UAG-ccsA
feature_type
igs
region
SSC
length_bp
73
variable_sites
2
pi
0.0278
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
69.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-ccsA_p1 CTTGAGCATCTCGGTTCGAA TCATCGACAACTAAGCTTATCA 50.0 36.4 58.0 55.0 204-205 bp 1.0 41.5
trnL-UAG-ccsA_p2 CTTGAGCATCTCGGTTCGAA TTTCATCGACAACTAAGCTT 50.0 35.0 58.0 52.9 206-207 bp 1.0 41.2
trnL-UAG-ccsA_p3 TAGACACGCTGCTCTTAGGA TCATCGACAACTAAGCTTATCA 50.0 36.4 57.9 55.0 231-232 bp 1.0 41.0
trnL-UAG-ccsA_p4 AAATTGGTAGACACGCTGCT TCATCGACAACTAAGCTTATCA 45.0 36.4 57.8 55.0 238-239 bp 1.0 41.0
trnL-UAG-ccsA_p5 GTAGACACGCTGCTCTTAGG TCATCGACAACTAAGCTTATCA 55.0 36.4 57.8 55.0 232-233 bp 1.0 41.0