Hotspot

rps19-rpl2

IRb · 81152-81205 · 54 bp

Back to Pachyphytum

Pi 0.037

MarkerSeek score 73.80

Primer available yes

Label rps19-rpl2

Feature Metadata

label_name: rps19-rpl2
feature_type: igs
region: IRb
length_bp: 54
variable_sites: 2
pi: 0.037
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl2_p1	GCCCGGGACCAAGTTACTAT	GGGCCCCAATTGGTAGAAAA	55.0	50.0	59.2	58.1	283 bp	1.0	70.8
rps19-rpl2_p2	GCCCGGGACCAAGTTACTAT	TTGATTCTTCGTCGCCGTAG	55.0	50.0	59.2	58.1	191 bp	1.0	70.8
rps19-rpl2_p3	GCCCGGGACCAAGTTACTAT	AGAAAAAGACCCACAACCCC	55.0	50.0	59.2	57.9	269 bp	1.0	70.6
rps19-rpl2_p4	GCCCGGGACCAAGTTACTAT	GAAAAAGACCCACAACCCCT	55.0	50.0	59.2	57.9	268 bp	1.0	70.6
rps19-rpl2_p5	GCCCGGGACCAAGTTACTAT	CACAACCCCTTGGGGTTATC	55.0	55.0	59.2	58.2	258 bp	1.0	70.1