Hotspot

psbF-psbE

LSC · 61525-61533 · 9 bp

Back to Pachyphytum

Pi 0.111

MarkerSeek score 87.50

Primer available yes

Label psbF-psbE

Feature Metadata

label_name: psbF-psbE
feature_type: igs
region: LSC
length_bp: 9
variable_sites: 1
pi: 0.111
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 87.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbF-psbE_p1	GTCCGTGAATAGCCAACCAT	TGTGTTTGGAAGTCCTCGAC	50.0	50.0	58.0	57.8	185 bp	1.0	78.3
psbF-psbE_p2	ACGGTAGGTACAGCTAGTCC	TGTGTTTGGAAGTCCTCGAC	55.0	50.0	58.0	57.8	201 bp	1.0	78.1
psbF-psbE_p3	GGTAGGTACAGCTAGTCCGT	TGTGTTTGGAAGTCCTCGAC	55.0	50.0	58.0	57.8	199 bp	1.0	78.1
psbF-psbE_p4	GTCCGTGAATAGCCAACCAT	GTTTGGAAGTCCTCGACCAA	50.0	50.0	58.0	57.8	182 bp	1.0	78.1
psbF-psbE_p5	GTCCGTGAATAGCCAACCAT	TTGGAAGTCCTCGACCAAAC	50.0	50.0	58.0	57.8	180 bp	1.0	78.1