Hotspot

accD-psaI

LSC · 56655-57214 · 560 bp

Back to Pachyphytum

Pi 0.0108

MarkerSeek score 65.00

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 560
variable_sites: 6
pi: 0.0108
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	CTCCACGCTTTCTTTCCCTT	AAAGAAGCCATTGCCATTGC	50.0	45.0	58.1	57.9	671-676 bp	1.0	79.7
accD-psaI_p2	CTCCACGCTTTCTTTCCCTT	ATTGCCATTGCCGGAAAAAC	50.0	45.0	58.1	58.2	662-667 bp	1.0	79.0
accD-psaI_p3	CTCCACGCTTTCTTTCCCTT	CATTGCCATTGCCGGAAAAA	50.0	45.0	58.1	58.2	663-668 bp	1.0	78.9
accD-psaI_p4	CTCCACGCTTTCTTTCCCTT	GCCATTGCCGGAAAAACTAG	50.0	50.0	58.1	57.7	659-664 bp	1.0	78.3
accD-psaI_p5	CTCCACGCTTTCTTTCCCTT	TGCCATTGCCGGAAAAACTA	50.0	45.0	58.1	58.4	660-665 bp	1.0	77.5