Hotspot

trnF-GAA-ndhJ

LSC · 46218-46865 · 648 bp

Back to Pachyphytum

Pi 0.0125

MarkerSeek score 66.80

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 648
variable_sites: 8
pi: 0.0125
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	ATCCCCGCTTGAAACGTATC	55.0	50.0	58.0	58.1	822-823 bp	1.0	80.9
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	ATCCCCGCTTGAAACGTATC	50.0	50.0	58.1	58.1	786-787 bp	1.0	80.6
trnF-GAA-ndhJ_p3	CTGGTAGAGCAGAGGACTGA	ATCCCCGCTTGAAACGTATC	55.0	50.0	57.9	58.1	810-811 bp	1.0	79.9
trnF-GAA-ndhJ_p4	GATACGGAAAGGATGGTCGG	ATCCCCGCTTGAAACGTATC	55.0	50.0	57.8	58.1	840-841 bp	1.0	79.7
trnF-GAA-ndhJ_p5	GAAAGGATGGTCGGGATAGC	ATCCCCGCTTGAAACGTATC	55.0	50.0	57.8	58.1	834-835 bp	1.0	79.6