Hotspot

psaA-ycf3

LSC · 40657-41331 · 675 bp

Back to Pachyphytum

Pi 0.00465

MarkerSeek score 65.70

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 675
variable_sites: 3
pi: 0.00465
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	CAGAGGCTTGGTTCGATCAA	50.0	50.0	57.9	57.9	838-868 bp	1.0	81.4
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	TTGAAGATCACGAGGCGTTT	50.0	45.0	57.9	57.8	752-782 bp	1.0	81.2
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	ATAGCACTTACTCCCGGGAA	50.0	50.0	57.9	57.8	794-824 bp	1.0	81.0
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	TAGCACTTACTCCCGGGAAT	50.0	50.0	57.9	57.8	793-823 bp	1.0	81.0
psaA-ycf3_p5	GCCCATTCCTCGAAAGAAGT	TTGGTTGAAGATCACGAGGC	50.0	50.0	57.9	58.2	756-786 bp	1.0	81.0