Hotspot

psbZ-trnG-UCC

LSC · 34583-35309 · 727 bp

Back to Pachyphytum

Pi 0.00567

MarkerSeek score 64.80

Primer available yes

Label psbZ-trnG-UCC

Feature Metadata

label_name: psbZ-trnG-UCC
feature_type: igs
region: LSC
length_bp: 727
variable_sites: 4
pi: 0.00567
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-UCC_p1	TTGCTTCTCCTGATGGTTGG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.8	57.8	884-932 bp	1.0	79.2
psbZ-trnG-UCC_p2	GCTTCTCCTGATGGTTGGTC	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	58.3	57.8	882-930 bp	1.0	78.8
psbZ-trnG-UCC_p3	AGTCTTTTTGGTGGGGATCC	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.4	57.8	814-862 bp	1.0	76.2
psbZ-trnG-UCC_p4	GTCTTTTTGGTGGGGATCCT	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.4	57.8	813-861 bp	1.0	76.2
psbZ-trnG-UCC_p5	TCTCCTGATGGTTGGTCAAC	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.1	57.8	879-927 bp	1.0	74.0