Hotspot

rps2-rpoC2

LSC · 15424-15733 · 310 bp

Back to Pachyphytum

Pi 0.0199

MarkerSeek score 56.10

Primer available yes

Label rps2-rpoC2

Feature Metadata

label_name: rps2-rpoC2
feature_type: igs
region: LSC
length_bp: 310
variable_sites: 6
pi: 0.0199
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 56.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rpoC2_p1	ACCAAAATGAACTCCCGCTT	ACATCAGAACAATCATTTCTGGG	45.0	39.1	58.0	57.1	412-423 bp	1.0	51.4
rps2-rpoC2_p2	AATGAACTCCCGCTTCCATC	ACATCAGAACAATCATTTCTGGG	50.0	39.1	58.0	57.1	407-418 bp	1.0	51.1
rps2-rpoC2_p3	CCCGCTTCCATCATCTCTTC	ACATCAGAACAATCATTTCTGGG	55.0	39.1	58.1	57.1	399-410 bp	1.0	50.5
rps2-rpoC2_p4	CCGCTTCCATCATCTCTTCC	ACATCAGAACAATCATTTCTGGG	55.0	39.1	58.1	57.1	398-409 bp	1.0	50.5
rps2-rpoC2_p5	ATATAAGGTGCCATTCGCGG	ACATCAGAACAATCATTTCTGGG	50.0	39.1	58.2	57.1	453-464 bp	1.0	49.6