Hotspot

rps16-trnQ-UUG

LSC · 5837-7239 · 1403 bp

Back to Pachyphytum

Pi 0.0116

MarkerSeek score 62.30

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1403
variable_sites: 16
pi: 0.0116
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1450-1476 bp	1.0	80.6
rps16-trnQ-UUG_p2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	1461-1487 bp	1.0	80.0
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	ATAAATCGAAGTGGGGCGTG	45.0	50.0	58.1	58.3	1509-1535 bp	1.0	78.4
rps16-trnQ-UUG_p4	CCACAGCTGATCATGTCCTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.6	58.1	1471-1497 bp	1.0	78.3
rps16-trnQ-UUG_p5	TCCACAGCTGATCATGTCCT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.4	58.1	1472-1498 bp	1.0	78.3