Hotspot

ycf1

SSC · 123656-129031 · 5376 bp

Back to Pachycladon

Pi 0.0107

MarkerSeek score 45.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5376
variable_sites: 112
pi: 0.0107
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCCATCGCAATTGAAAACGC	GAAACCGTGGGTCATACCAA	45.0	50.0	58.0	57.8	353-359 bp	1.0	82.8
ycf1_p2	TCCATCGCAATTGAAAACGC	CCGATTGCATGGGCTAGATT	45.0	50.0	58.0	58.1	958-964 bp	0.8	74.1
ycf1_p3	ACAGACAAACGGACAGGTTC	CCGATTGCATGGGCTAGATT	50.0	50.0	58.1	58.1	1160-1166 bp	0.8	73.6
ycf1_p4	CAGACAAACGGACAGGTTCA	CCGATTGCATGGGCTAGATT	50.0	50.0	58.1	58.1	1159-1165 bp	0.8	73.5
ycf1_p5	TCCATCGCAATTGAAAACGC	GGGTATAGGGGAAATTCGGC	45.0	55.0	58.0	57.8	650-656 bp	0.8	73.3