Hotspot

rps16

LSC · 4995-6148 · 1154 bp

Back to Pachycladon

Pi 0.0116

MarkerSeek score 57.60

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1154
variable_sites: 27
pi: 0.0116
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	ACGAGTCTTTCACATCCTCT	TCAATTACACAAACTAGAGGAATGT	45.0	32.0	55.9	56.5	1213-1235 bp	1.0	42.9
rps16_p2	ACGAGTCTTTCACATCCTCTT	TCAATTACACAAACTAGAGGAATGT	42.9	32.0	56.7	56.5	1213-1235 bp	1.0	42.9
rps16_p3	ACGAGTCTTTCACATCCTCT	TGAATCAATTACACAAACTAGAGGA	45.0	32.0	55.9	56.2	1217-1239 bp	1.0	42.9
rps16_p4	ACGAGTCTTTCACATCCTCT	CAATTACACAAACTAGAGGAATGT	45.0	33.3	55.9	55.1	1212-1234 bp	1.0	42.9
rps16_p5	ACGAGTCTTTCACATCCTCT	TACACAAACTAGAGGAATGTT	45.0	33.3	55.9	52.1	1208-1230 bp	1.0	42.9