Hotspot

rpl32-trnL

SSC · 113257-114048 · 792 bp

Back to Pachycladon

Pi 0.0235

MarkerSeek score 63.00

Primer available yes

Label rpl32-trnL

Feature Metadata

label_name: rpl32-trnL
feature_type: igs
region: SSC
length_bp: 792
variable_sites: 33
pi: 0.0235
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL_p1	GGAAGGGATATTGGACATCGT	TCCTAAGAGCAGCGTGTCTA	47.6	50.0	57.2	57.9	911-948 bp	1.0	69.7
rpl32-trnL_p2	GGAAGGGATATTGGACATCGT	TGCAGACTGAGGCATTTTGT	47.6	45.0	57.2	58.0	1449-1486 bp	1.0	69.3
rpl32-trnL_p3	GGAAGGGATATTGGACATCGT	AGCAGCGTGTCTACCAATTT	47.6	45.0	57.2	57.8	904-941 bp	1.0	69.3
rpl32-trnL_p4	GGAAGGGATATTGGACATCGT	CTAAGAGCAGCGTGTCTACC	47.6	55.0	57.2	57.8	909-946 bp	1.0	69.2
rpl32-trnL_p5	GGAAGGGATATTGGACATCGT	TAACCACTGGGACTGGAGAG	47.6	55.0	57.2	58.1	1482-1519 bp	1.0	68.9