Hotspot

clpP-psbB

LSC · 71223-71709 · 487 bp

Back to Pachycladon

Pi 0.0103

MarkerSeek score 62.00

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 487
variable_sites: 10
pi: 0.0103
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	CTCCAGGACTTCGAAAAGGT	ACAGAAAGTAAACGACCGGG	50.0	50.0	57.2	57.8	590-596 bp	1.0	74.0
clpP-psbB_p2	CTCCAGGACTTCGAAAAGGT	CGATACCAAGGCAAACCCAT	50.0	50.0	57.2	58.2	545-551 bp	1.0	73.6
clpP-psbB_p3	CTCCAGGACTTCGAAAAGGT	CAGAAAGTAAACGACCGGGA	50.0	50.0	57.2	57.6	589-595 bp	1.0	71.7
clpP-psbB_p4	TCTCCAGGACTTCGAAAAGG	ACAGAAAGTAAACGACCGGG	50.0	50.0	56.9	57.8	591-597 bp	1.0	71.7
clpP-psbB_p5	CTCCAGGACTTCGAAAAGGT	ACACGATACCAAGGCAAACC	50.0	50.0	57.2	58.5	548-554 bp	1.0	71.7