Hotspot

ndhK-ndhC

LSC · 49271-49336 · 66 bp

Back to Pachycladon

Pi 0.0626

MarkerSeek score 73.20

Primer available yes

Label ndhK-ndhC

Feature Metadata

label_name: ndhK-ndhC
feature_type: igs
region: LSC
length_bp: 66
variable_sites: 8
pi: 0.0626
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK-ndhC_p1	GGCTGGAAAGTCTTGACCAA	CATGGCGAAAAGGAGCATTG	50.0	50.0	58.0	58.1	197-220 bp	1.0	83.1
ndhK-ndhC_p2	AGGCTGGAAAGTCTTGACCA	CATGGCGAAAAGGAGCATTG	50.0	50.0	58.9	58.1	198-221 bp	1.0	76.3
ndhK-ndhC_p3	GGCTGGAAAGTCTTGACCAA	GCGAAAAGGAGCATTGGAAT	50.0	45.0	58.0	57.1	193-216 bp	1.0	76.2
ndhK-ndhC_p4	GGCTGGAAAGTCTTGACCAA	TATGCATGGCGAAAAGGAGC	50.0	50.0	58.0	59.0	201-224 bp	1.0	75.9
ndhK-ndhC_p5	ACCATAAAGAAGCGGCCATA	CATGGCGAAAAGGAGCATTG	45.0	50.0	57.0	58.1	217-240 bp	1.0	74.7