Hotspot

psbM-trnD

LSC · 28426-29423 · 998 bp

Back to Pachycladon

Pi 0.0132

MarkerSeek score 62.00

Primer available yes

Label psbM-trnD

Feature Metadata

label_name: psbM-trnD
feature_type: igs
region: LSC
length_bp: 998
variable_sites: 26
pi: 0.0132
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD_p1	AGGAACGAGAATGAAGAGTGC	TTCAATTGGTCAGAGCACCG	47.6	50.0	58.0	58.5	1110-1128 bp	1.0	70.8
psbM-trnD_p2	ACGAGAATGAAGAGTGCAGT	TTCAATTGGTCAGAGCACCG	45.0	50.0	56.9	58.5	1106-1124 bp	1.0	70.2
psbM-trnD_p3	GGAACGAGAATGAAGAGTGCA	TTCAATTGGTCAGAGCACCG	47.6	50.0	58.3	58.5	1109-1127 bp	1.0	68.6
psbM-trnD_p4	AACGAGAATGAAGAGTGCAGT	TTCAATTGGTCAGAGCACCG	42.9	50.0	57.6	58.5	1107-1125 bp	1.0	68.0
psbM-trnD_p5	GGAACGAGAATGAAGAGTGC	TTCAATTGGTCAGAGCACCG	50.0	50.0	56.5	58.5	1109-1127 bp	1.0	67.5