Hotspot

rpoB-trnC

LSC · 26205-27323 · 1119 bp

Back to Pachycladon

Pi 0.0134

MarkerSeek score 58.30

Primer available yes

Label rpoB-trnC

Feature Metadata

label_name: rpoB-trnC
feature_type: igs
region: LSC
length_bp: 1119
variable_sites: 28
pi: 0.0134
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC_p1	GTTCCCTCTTTTCCATCCCC	CCGGATTTGAACTGGGGAAA	55.0	50.0	57.9	58.1	1204-1211 bp	1.0	81.4
rpoB-trnC_p2	CCCTCTTTTCCATCCCCAAG	CCGGATTTGAACTGGGGAAA	55.0	50.0	57.9	58.1	1201-1208 bp	1.0	81.3
rpoB-trnC_p3	CTCTTTTCCATCCCCAAGCA	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.8	58.1	1199-1206 bp	1.0	80.8
rpoB-trnC_p4	TCCCTCTTTTCCATCCCCAA	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.2	58.1	1202-1209 bp	1.0	80.7
rpoB-trnC_p5	TTCCCTCTTTTCCATCCCCA	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.2	58.1	1203-1210 bp	1.0	80.7