Hotspot

rps16-trnQ

LSC · 6149-6568 · 420 bp

Back to Pachycladon

Pi 0.0228

MarkerSeek score 67.80

Primer available yes

Label rps16-trnQ

Feature Metadata

label_name: rps16-trnQ
feature_type: igs
region: LSC
length_bp: 420
variable_sites: 18
pi: 0.0228
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	440-523 bp	1.0	85.2
rps16-trnQ_p2	CCTTCAATTCAAGTCGCACG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.8	58.1	450-533 bp	1.0	84.4
rps16-trnQ_p3	CCACAGCAGATCATGTCCTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.6	58.1	466-549 bp	1.0	82.9
rps16-trnQ_p4	TCCACAGCAGATCATGTCCT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.4	58.1	467-550 bp	1.0	82.8
rps16-trnQ_p5	ATCCACAGCAGATCATGTCC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.4	58.1	468-551 bp	1.0	81.1