Hotspot

trnK-rps16

LSC · 4329-4994 · 666 bp

Back to Pachycladon

Pi 0.0214

MarkerSeek score 67.00

Primer available yes

Label trnK-rps16

Feature Metadata

label_name: trnK-rps16
feature_type: igs
region: LSC
length_bp: 666
variable_sites: 29
pi: 0.0214
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-rps16_p1	TACCGTTGAGTTAGCAACCC	ACGGAATTAAGTCTTAATAAAACGA	50.0	28.0	57.5	55.1	688-790 bp	0.8	37.5
trnK-rps16_p2	AAAGCCGAGTACTCTACCGT	ACGGAATTAAGTCTTAATAAAACGA	50.0	28.0	58.2	55.1	702-804 bp	0.8	37.4
trnK-rps16_p3	AAGCCGAGTACTCTACCGTT	ACGGAATTAAGTCTTAATAAAACGA	50.0	28.0	58.2	55.1	701-803 bp	0.8	37.4
trnK-rps16_p4	AAAGCCGAGTACTCTACCGT	ACGGAATTAAGTCTTAATAAAACGAA	50.0	26.9	58.2	55.8	702-804 bp	0.8	37.4
trnK-rps16_p5	AAGCCGAGTACTCTACCGTT	ACGGAATTAAGTCTTAATAAAACGAA	50.0	26.9	58.2	55.8	701-803 bp	0.8	37.4