Hotspot

trnH-psbA

LSC · 84-357 · 274 bp

Back to Pachycladon

Pi 0.0418

MarkerSeek score 85.90

Primer available yes

Label trnH-psbA

Feature Metadata

label_name: trnH-psbA
feature_type: igs
region: LSC
length_bp: 274
variable_sites: 23
pi: 0.0418
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 85.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-psbA_p1	CTGCCTTAATCCACTTGGCT	GCTGTTGAGGCTCCATCTAC	50.0	55.0	57.9	58.1	330-396 bp	1.0	86.0
trnH-psbA_p2	ACTGCCTTAATCCACTTGGC	GCTGTTGAGGCTCCATCTAC	50.0	55.0	58.2	58.1	331-397 bp	1.0	85.8
trnH-psbA_p3	CTGCCTTAATCCACTTGGCT	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	345-411 bp	1.0	85.6
trnH-psbA_p4	CTGCCTTAATCCACTTGGCT	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	348-414 bp	1.0	85.5
trnH-psbA_p5	ACTGCCTTAATCCACTTGGC	CCTCTAGACCTAGCTGCTGT	50.0	55.0	58.2	57.9	346-412 bp	1.0	85.4