Hotspot

ndhI

SSC · 122063-122566 · 504 bp

Back to Pachira

Pi 0.515

MarkerSeek score 77.60

Primer available yes

Label ndhI

Feature Metadata

label_name: ndhI
feature_type: gene
region: SSC
length_bp: 504
variable_sites: 210
pi: 0.515
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 77.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI_p1	AGGGAGAAAGGCCTCATTCT	GCGTCAGCCAATAGGGTTTA	50.0	50.0	58.0	58.0	1162 bp	0.5	59.6
ndhI_p2	AATCGTGTATTGGTCCAGGC	GCGTCAGCCAATAGGGTTTA	50.0	50.0	58.0	58.0	1503 bp	0.5	59.4
ndhI_p3	AGAGAAAGGGAGAAAGGCCT	GCGTCAGCCAATAGGGTTTA	50.0	50.0	57.9	58.0	1168 bp	0.5	59.3
ndhI_p4	AGGGAGAAAGGCCTCATTCT	TACCAGAAGCCGAGGAAGAA	50.0	50.0	58.0	58.1	1091 bp	0.5	59.3
ndhI_p5	AGGGAGAAAGGCCTCATTCT	TTACCAGAAGCCGAGGAAGA	50.0	50.0	58.0	58.1	1092 bp	0.5	59.3