Hotspot

ndhF

SSC · 112501-114711 · 2211 bp

Back to Pachira

Pi 0.478

MarkerSeek score 80.10

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2211
variable_sites: 920
pi: 0.478
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CCTTGTTCCTACATGACCCG	AGAGCCGGTGAATCAGAAAC	55.0	50.0	58.0	57.9	2733 bp	0.5	59.0
ndhF_p2	GGTGACGATACTGTAGGGGA	AGACAATCGATCAGTTCCGC	55.0	50.0	57.9	58.1	2850 bp	0.5	58.9
ndhF_p3	TGTCCCCTCCAGTCAAGAAT	AGAGCCGGTGAATCAGAAAC	50.0	50.0	58.0	57.9	2591 bp	0.5	58.8
ndhF_p4	GTCAAGTGGAAGTGCAGTGA	AGAGCCGGTGAATCAGAAAC	50.0	50.0	58.0	57.9	2794 bp	0.5	58.8
ndhF_p5	CAGCTGAGGCATCCTAACAG	AGAGCCGGTGAATCAGAAAC	55.0	50.0	58.0	57.9	2768 bp	0.5	58.8