Hotspot

ndhD

SSC · 118449-119951 · 1503 bp

Back to Pachira

Pi 0.475

MarkerSeek score 79.40

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 572
pi: 0.475
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.80
markerseek_score: 79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	TTGGACCGTATTCGCGATTT	CAAATTGCTTCCGCTCCAAG	45.0	50.0	58.0	58.0	2242 bp	0.5	59.8
ndhD_p2	GTAAATTCCGCACTTGTGGC	CAAATTGCTTCCGCTCCAAG	50.0	50.0	58.0	58.0	2187 bp	0.5	59.7
ndhD_p3	TTGGACCGTATTCGCGATTT	AGATGTGAATCCGCTTGTCC	45.0	50.0	58.0	58.0	2197 bp	0.5	59.7
ndhD_p4	GTAAATTCCGCACTTGTGGC	AGATGTGAATCCGCTTGTCC	50.0	50.0	58.0	58.0	2142 bp	0.5	59.6
ndhD_p5	TTACTTGGACCGTATTCGCG	CAAATTGCTTCCGCTCCAAG	50.0	50.0	58.1	58.0	2246 bp	0.5	59.2