Hotspot

ccsA

SSC · 117178-118149 · 972 bp

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Pi 0.454

MarkerSeek score 78.40

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 972
variable_sites: 337
pi: 0.454
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.76
markerseek_score: 78.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	TCCTTCCCTTGCTTTCAACC	CGAAATGGGCGGAATGTCTA	50.0	50.0	58.0	58.1	2390 bp	0.5	59.5
ccsA_p2	TCCTTCCCTTGCTTTCAACC	TGGGCGGAATGTCTATTTCG	50.0	50.0	58.0	58.1	2385 bp	0.5	59.5
ccsA_p3	GTGAAATCGGTAGACACGCT	CGAAATGGGCGGAATGTCTA	50.0	50.0	58.0	58.1	1859 bp	0.5	59.5
ccsA_p4	GTGAAATCGGTAGACACGCT	TGGGCGGAATGTCTATTTCG	50.0	50.0	58.0	58.1	1854 bp	0.5	59.5
ccsA_p5	TGAAATCGGTAGACACGCTG	TGGGCGGAATGTCTATTTCG	50.0	50.0	58.0	58.1	1853 bp	0.5	59.5