Hotspot

ycf1-trnN-GUU

SSC · 132485-132801 · 317 bp

Back to Pachira

Pi 0.475

MarkerSeek score 67.40

Primer available yes

Label ycf1-trnN-GUU

Feature Metadata

label_name: ycf1-trnN-GUU
feature_type: igs
region: SSC
length_bp: 317
variable_sites: 84
pi: 0.475
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.56
markerseek_score: 67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-trnN-GUU_p1	AATGGGTGACGGTATTCTGC	GGGTTACCTGCTCTTAACGG	50.0	55.0	58.0	58.0	1190 bp	0.5	59.5
ycf1-trnN-GUU_p2	CCTTCTTCCATAACCCGAGC	GGGTTACCTGCTCTTAACGG	55.0	55.0	58.0	58.0	625 bp	0.5	59.5
ycf1-trnN-GUU_p3	AATGGGTGACGGTATTCTGC	AACGGTAAAAGCGAACCCTT	50.0	45.0	58.0	58.0	1175 bp	0.5	59.5
ycf1-trnN-GUU_p4	AATGGGTGACGGTATTCTGC	ACGGTAAAAGCGAACCCTTT	50.0	45.0	58.0	58.0	1174 bp	0.5	59.5
ycf1-trnN-GUU_p5	CCTTCTTCCATAACCCGAGC	ACGGTAAAAGCGAACCCTTT	55.0	45.0	58.0	58.0	609 bp	0.5	59.4