Hotspot

ndhG-ndhI

SSC · 121640-122062 · 423 bp

Back to Pachira

Pi 0.461

MarkerSeek score 76.90

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 423
variable_sites: 164
pi: 0.461
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	AATCGTGTATTGGTCCAGGC	TTATGGCCACCAAACAGTCC	50.0	50.0	58.0	58.1	926 bp	0.5	59.0
ndhG-ndhI_p2	AATCGTGTATTGGTCCAGGC	AATCAAATTGCTTTGGGCCG	50.0	45.0	58.0	57.9	544 bp	0.5	58.9
ndhG-ndhI_p3	CGAAGTAAGGCCATTCCCAA	TTATGGCCACCAAACAGTCC	50.0	50.0	57.9	58.1	1201 bp	0.5	58.4
ndhG-ndhI_p4	AACGAAGTAAGGCCATTCCC	TTATGGCCACCAAACAGTCC	50.0	50.0	57.9	58.1	1203 bp	0.5	58.3
ndhG-ndhI_p5	AATCGTGTATTGGTCCAGGC	AGCAGCAAGGTACATTGGTC	50.0	50.0	58.0	58.2	905 bp	0.5	58.3