Hotspot

psaC-ndhE

SSC · 120328-120574 · 247 bp

Back to Pachira

Pi 0.517

MarkerSeek score 74.20

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 247
variable_sites: 107
pi: 0.517
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 74.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	CTTCCGCCAGCTGGATTAAT	TTGCTGTGGCTCGTCAATAA	50.0	45.0	58.0	57.8	1376 bp	0.5	58.4
psaC-ndhE_p2	GGACAAGCGGATTCACATCT	TTGCTGTGGCTCGTCAATAA	50.0	45.0	58.0	57.8	977 bp	0.5	58.3
psaC-ndhE_p3	CGCCAGCTGGATTAATGGAT	TTGCTGTGGCTCGTCAATAA	50.0	45.0	58.1	57.8	1372 bp	0.5	57.8
psaC-ndhE_p4	CGAATCTCGAGTAACTGGCC	TTGCTGTGGCTCGTCAATAA	55.0	45.0	58.2	57.8	1510 bp	0.5	57.3
psaC-ndhE_p5	TTCTGTTCTTGGAGCGGAAG	TTGCTGTGGCTCGTCAATAA	50.0	45.0	57.8	57.8	939 bp	0.5	57.2