Hotspot

ccsA-ndhD

SSC · 118150-118448 · 299 bp

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Pi 0.451

MarkerSeek score 77.40

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 299
variable_sites: 128
pi: 0.451
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TTGGACCGTATTCGCGATTT	CGAAATGGGCGGAATGTCTA	45.0	50.0	58.0	58.1	892 bp	0.5	59.5
ccsA-ndhD_p2	TTGGACCGTATTCGCGATTT	TGGGCGGAATGTCTATTTCG	45.0	50.0	58.0	58.1	887 bp	0.5	59.5
ccsA-ndhD_p3	GTAAATTCCGCACTTGTGGC	CGAAATGGGCGGAATGTCTA	50.0	50.0	58.0	58.1	837 bp	0.5	59.4
ccsA-ndhD_p4	GTAAATTCCGCACTTGTGGC	TGGGCGGAATGTCTATTTCG	50.0	50.0	58.0	58.1	832 bp	0.5	59.4
ccsA-ndhD_p5	TTACTTGGACCGTATTCGCG	TGGGCGGAATGTCTATTTCG	50.0	50.0	58.1	58.1	891 bp	0.5	58.9