Hotspot

rpl2

Genome · 80337-81870 · 1534 bp

Back to Oxytropis

Pi 0.0428

MarkerSeek score 84.60

Primer available yes

Label rpl2

Feature Metadata

label_name: rpl2
feature_type: gene
region: Genome
length_bp: 1534
variable_sites: 661
pi: 0.0428
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 84.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2_p1	TGTATTGTAAAGACGAAGAAACA	CGCTTCAACCGGGTTATTCT	30.4	50.0	54.3	58.3	1662-1684 bp	0.857143	36.1
rpl2_p2	TGTATTGTAAAGACGAAGAAACAA	CGCTTCAACCGGGTTATTCT	29.2	50.0	55.0	58.3	1662-1684 bp	0.857143	36.1
rpl2_p3	TGTATTGTAAAGACGAAGAAACA	ATCATTACGCTTCAACCGGG	30.4	50.0	54.3	58.3	1669-1691 bp	0.857143	36.0
rpl2_p4	TTGTATTGTAAAGACGAAGAAACA	CGCTTCAACCGGGTTATTCT	29.2	50.0	55.0	58.3	1663-1685 bp	0.857143	36.0
rpl2_p5	TGTATTGTAAAGACGAAGAAACA	ACGCTTCAACCGGGTTATTC	30.4	50.0	54.3	58.6	1663-1685 bp	0.857143	36.0