Hotspot

ndhF

Genome · 119697-121931 · 2235 bp

Back to Oxytropis

Pi 0.0455

MarkerSeek score 75.00

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: Genome
length_bp: 2235
variable_sites: 1025
pi: 0.0455
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACCGAACCCTTTACAAAAGAGA	CATAGAGATGGAATAGTACATTATATT	40.9	25.9	57.6	52.7	2357-2383 bp	0.952381	39.6
ndhF_p2	ACCGAACCCTTTACAAAAGAG	CATAGAGATGGAATAGTACATTATATT	42.9	25.9	56.1	52.7	2357-2383 bp	0.952381	39.6
ndhF_p3	ACCGAACCCTTTACAAAAGAGAT	CATAGAGATGGAATAGTACATTATATT	39.1	25.9	58.0	52.7	2357-2383 bp	0.952381	39.6
ndhF_p4	AACCGAACCCTTTACAAAAGAG	CATAGAGATGGAATAGTACATTATATT	40.9	25.9	56.8	52.7	2358-2384 bp	0.952381	39.6
ndhF_p5	CCGAACCCTTTACAAAAGAGA	CATAGAGATGGAATAGTACATTATATT	42.9	25.9	55.8	52.7	2356-2382 bp	0.952381	39.6