Hotspot

trnL-TAG-rpl32

Genome · 118136-118814 · 679 bp

Back to Oxytropis

Pi 0.0472
MarkerSeek score 82.80
Primer available yes
Label trnL-TAG-rpl32

Feature Metadata

label_name
trnL-TAG-rpl32
feature_type
igs
region
Genome
length_bp
679
variable_sites
274
pi
0.0472
unique_haplotype_count
21
primer_available
yes
alignment_reliability
0.99
markerseek_score
82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-TAG-rpl32_p1 TCCTAAGAGCAGCGTGTCTA TGGACAGCATTGAAAGCCTT 50.0 45.0 57.9 58.0 660-816 bp 0.952381 85.0
trnL-TAG-rpl32_p2 TCCTAAGAGCAGCGTGTCTA TTGGACAGCATTGAAAGCCT 50.0 45.0 57.9 58.0 661-817 bp 0.952381 85.0
trnL-TAG-rpl32_p3 AGCAGCGTGTCTACCAATTT TGGACAGCATTGAAAGCCTT 45.0 45.0 57.8 58.0 653-809 bp 0.952381 84.5
trnL-TAG-rpl32_p4 ATGGGATTGTAGAGCGGGTA TGGACAGCATTGAAAGCCTT 50.0 45.0 57.9 58.0 1239-1389 bp 0.952381 84.3
trnL-TAG-rpl32_p5 ATGGGATTGTAGAGCGGGTA TTGGACAGCATTGAAAGCCT 50.0 45.0 57.9 58.0 1240-1390 bp 0.952381 84.3