Hotspot

ycf1-rps15

Genome · 108181-108625 · 445 bp

Back to Oxytropis

Pi 0.0487

MarkerSeek score 86.90

Primer available yes

Label ycf1-rps15

Feature Metadata

label_name: ycf1-rps15
feature_type: igs
region: Genome
length_bp: 445
variable_sites: 200
pi: 0.0487
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 86.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-rps15_p1	TTGATACCCAGAACGGAAGC	TCAACTGATCCAGGGTTTTCT	50.0	42.9	57.6	56.8	575-597 bp	0.952381	64.2
ycf1-rps15_p2	TTGATACCCAGAACGGAAGC	TGAAATTCAACTGATCCAGGGT	50.0	40.9	57.6	57.6	581-603 bp	0.952381	62.4
ycf1-rps15_p3	TTGATACCCAGAACGGAAGC	TCAACTGATCCAGGGTTTTCTT	50.0	40.9	57.6	57.5	575-597 bp	0.952381	61.9
ycf1-rps15_p4	TTGATACCCAGAACGGAAGC	TTCAACTGATCCAGGGTTTTCT	50.0	40.9	57.6	57.5	576-598 bp	0.952381	61.9
ycf1-rps15_p5	TTGATACCCAGAACGGAAGC	ACTGATCCAGGGTTTTCTTCT	50.0	42.9	57.6	56.5	572-594 bp	0.952381	61.9