Hotspot

rps12-trnV-GAC

Genome · 92901-94469 · 1569 bp

Back to Oxytropis

Pi 0.045

MarkerSeek score 85.00

Primer available yes

Label rps12-trnV-GAC

Feature Metadata

label_name: rps12-trnV-GAC
feature_type: igs
region: Genome
length_bp: 1569
variable_sites: 671
pi: 0.045
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 85.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-trnV-GAC_p1	GAGGTTAAGCGTACTCTGGC	TTCGAACCGATGACTTCCAC	55.0	50.0	58.1	57.9	1689-1799 bp	0.952381	81.8
rps12-trnV-GAC_p2	GGTTAAGCGTACTCTGGCAA	TTCGAACCGATGACTTCCAC	50.0	50.0	57.9	57.9	1687-1797 bp	0.952381	81.7
rps12-trnV-GAC_p3	GAGGTTAAGCGTACTCTGGC	GATGACTTCCACCACGTCAA	55.0	50.0	58.1	57.8	1681-1791 bp	0.952381	81.1
rps12-trnV-GAC_p4	GAGGTTAAGCGTACTCTGGC	ATGACTTCCACCACGTCAAG	55.0	50.0	58.1	57.8	1680-1790 bp	0.952381	81.1
rps12-trnV-GAC_p5	GGTTAAGCGTACTCTGGCAA	GATGACTTCCACCACGTCAA	50.0	50.0	57.9	57.8	1679-1789 bp	0.952381	81.0