Hotspot

trnL-CAA-ndhB

Genome · 89071-89637 · 567 bp

Back to Oxytropis

Pi 0.0533

MarkerSeek score 85.40

Primer available yes

Label trnL-CAA-ndhB

Feature Metadata

label_name: trnL-CAA-ndhB
feature_type: igs
region: Genome
length_bp: 567
variable_sites: 267
pi: 0.0533
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 85.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-ndhB_p1	GTCTCGCGTGTCTACCATTT	CCAGGAATATCAATGAACCCCA	50.0	45.5	58.0	57.8	624-670 bp	0.952381	68.5
trnL-CAA-ndhB_p2	CTCGCGTGTCTACCATTTCA	CCAGGAATATCAATGAACCCCA	50.0	45.5	58.0	57.8	622-668 bp	0.952381	68.5
trnL-CAA-ndhB_p3	GTCTCGCGTGTCTACCATTT	ACCAGGAATATCAATGAACCCC	50.0	45.5	58.0	57.8	625-671 bp	0.952381	68.5
trnL-CAA-ndhB_p4	CTCGCGTGTCTACCATTTCA	ACCAGGAATATCAATGAACCCC	50.0	45.5	58.0	57.8	623-669 bp	0.952381	68.5
trnL-CAA-ndhB_p5	TACCATTTCACCACCAAGGC	CCAGGAATATCAATGAACCCCA	50.0	45.5	58.1	57.8	612-658 bp	0.952381	68.1