Hotspot

trnQ-UUG-accD

Genome · 53902-54680 · 779 bp

Back to Oxytropis

Pi 0.0378

MarkerSeek score 82.80

Primer available yes

Label trnQ-UUG-accD

Feature Metadata

label_name: trnQ-UUG-accD
feature_type: igs
region: Genome
length_bp: 779
variable_sites: 226
pi: 0.0378
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-accD_p1	GAGGTTCGAATCCTTCCGTC	TTCCGTATGTTCCTGCGATC	55.0	50.0	58.1	57.8	882-1245 bp	0.904762	82.1
trnQ-UUG-accD_p2	GAGGTTCGAATCCTTCCGTC	GTATGTTCCTGCGATCCACT	55.0	50.0	58.1	57.4	878-1511 bp	0.952381	81.6
trnQ-UUG-accD_p3	GAGGTTCGAATCCTTCCGTC	CGTATGTTCCTGCGATCCAC	55.0	55.0	58.1	58.8	879-1512 bp	0.952381	80.1
trnQ-UUG-accD_p4	GAGGTTCGAATCCTTCCGTC	AATTCCGTATGTTCCTGCGA	55.0	45.0	58.1	57.3	884-1247 bp	0.904762	78.3
trnQ-UUG-accD_p5	TTCGGAGGTTCGAATCCTTC	TTCCGTATGTTCCTGCGATC	50.0	50.0	57.3	57.8	886-1249 bp	0.904762	77.3