Hotspot

trnC-GCA-rpoB

Genome · 33572-34620 · 1049 bp

Back to Oxytropis

Pi 0.0326

MarkerSeek score 82.60

Primer available yes

Label trnC-GCA-rpoB

Feature Metadata

label_name: trnC-GCA-rpoB
feature_type: igs
region: Genome
length_bp: 1049
variable_sites: 298
pi: 0.0326
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-rpoB_p1	CTTACCACTCGGCCATATCG	TTCATTTCCAACCCCAAGCA	55.0	45.0	57.9	57.9	1088-1097 bp	0.952381	83.9
trnC-GCA-rpoB_p2	CCGGATTTGAACTGGGGAAA	TTCATTTCCAACCCCAAGCA	50.0	45.0	58.1	57.9	1128-1137 bp	0.952381	83.7
trnC-GCA-rpoB_p3	CTTACCACTCGGCCATATCG	TACGCCCCGATCAATGAATC	55.0	50.0	57.9	57.8	1160-1169 bp	0.952381	83.2
trnC-GCA-rpoB_p4	CCGGATTTGAACTGGGGAAA	TACGCCCCGATCAATGAATC	50.0	50.0	58.1	57.8	1200-1209 bp	0.952381	83.0
trnC-GCA-rpoB_p5	CTTACCACTCGGCCATATCG	AAGTTCTTCTGTTACGCCCC	55.0	50.0	57.9	57.8	1172-1181 bp	0.904762	80.3