Hotspot

trnD-GTC-psbM

Genome · 31077-31487 · 411 bp

Back to Oxytropis

Pi 0.0676

MarkerSeek score 76.30

Primer available yes

Label trnD-GTC-psbM

Feature Metadata

label_name: trnD-GTC-psbM
feature_type: igs
region: Genome
length_bp: 411
variable_sites: 152
pi: 0.0676
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 76.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GTC-psbM_p1	CATAGTAGAATGGCGGTCGG	CCCCGGATCATCTGTCAATT	55.0	50.0	57.9	57.4	1709-1731 bp	0.857143	75.5
trnD-GTC-psbM_p2	CATAGTAGAATGGCGGTCGG	TCGATGAACTGGTTACGACC	55.0	50.0	57.9	57.4	1775-1797 bp	0.809524	72.5
trnD-GTC-psbM_p3	AAGTATGCCCCCATCGTCTA	TCGATGAACTGGTTACGACC	50.0	50.0	57.9	57.4	1753-1775 bp	0.809524	72.2
trnD-GTC-psbM_p4	GAGTAATCCGTGCCGATCTC	CCCCGGATCATCTGTCAATT	55.0	50.0	57.9	57.4	1221-1246 bp	0.52381	61.7
trnD-GTC-psbM_p5	GAGTAATCCGTGCCGATCTC	TCGATGAACTGGTTACGACC	55.0	50.0	57.9	57.4	1287-1312 bp	0.47619	58.5