Hotspot

psbA

LSC · 305-1366 · 1062 bp

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Pi 0.00235

MarkerSeek score 33.10

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 5
pi: 0.00235
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 33.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	GATCCACTTGGCTACATCCG	AACACAAAACTACTGGCCGT	55.0	45.0	58.1	58.0	2049-2058 bp	1.0	80.2
psbA_p2	GATCCACTTGGCTACATCCG	ACAAAACTACTGGCCGTGTT	55.0	45.0	58.1	58.0	2046-2055 bp	1.0	80.2
psbA_p3	CAATCCACTGCCTTGATCCA	AACACAAAACTACTGGCCGT	50.0	45.0	57.9	58.0	2063-2072 bp	1.0	80.1
psbA_p4	CAATCCACTGCCTTGATCCA	ACAAAACTACTGGCCGTGTT	50.0	45.0	57.9	58.0	2060-2069 bp	1.0	80.1
psbA_p5	AATCCACTGCCTTGATCCAC	ACAAAACTACTGGCCGTGTT	50.0	45.0	57.9	58.0	2059-2068 bp	1.0	80.1