Hotspot

ndhF

SSC · 112514-114751 · 2238 bp

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Pi 0.00737

MarkerSeek score 51.80

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2238
variable_sites: 32
pi: 0.00737
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TCCAAAATGTCAATCCAGAGT	GAGTTGAGAAAGAATAATTCAGA	38.1	30.4	55.1	52.3	2309-2315 bp	1.0	41.3
ndhF_p2	CATCCAAAATGTCAATCCAGAGT	GAGTTGAGAAAGAATAATTCAGA	39.1	30.4	57.1	52.3	2311-2317 bp	1.0	41.3
ndhF_p3	TCCAACAAGGAAGAATTGTCT	GAGTTGAGAAAGAATAATTCAGA	38.1	30.4	55.0	52.3	2366-2372 bp	1.0	41.3
ndhF_p4	TCCAAAATGTCAATCCAGAGT	GAGTTGAGAAAGAATAATTCAGAA	38.1	29.2	55.1	53.1	2309-2315 bp	1.0	41.3
ndhF_p5	CATCCAAAATGTCAATCCAGAGT	GAGTTGAGAAAGAATAATTCAGAA	39.1	29.2	57.1	53.1	2311-2317 bp	1.0	41.3