Hotspot

ccsA

SSC · 116324-117274 · 951 bp

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Pi 0.00526

MarkerSeek score 39.80

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 951
variable_sites: 10
pi: 0.00526
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 39.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GTGAAATCGGTAGACACGCT	TCAATGGCTTCCCTTGCATT	50.0	45.0	58.0	58.1	1811-1815 bp	1.0	81.0
ccsA_p2	TGAAATCGGTAGACACGCTG	TCAATGGCTTCCCTTGCATT	50.0	45.0	58.0	58.1	1810-1814 bp	1.0	81.0
ccsA_p3	GTGAAATCGGTAGACACGCT	TGGTTCTCGACATCTTCCCT	50.0	50.0	58.0	58.1	1285-1287 bp	1.0	81.0
ccsA_p4	TGAAATCGGTAGACACGCTG	TGGTTCTCGACATCTTCCCT	50.0	50.0	58.0	58.1	1284-1286 bp	1.0	81.0
ccsA_p5	GTGAAATCGGTAGACACGCT	TTTTGATTCTGGGCCGAGAG	50.0	50.0	58.0	57.9	1593-1597 bp	1.0	80.7