Hotspot

ndhF-rpl32

SSC · 114752-115680 · 929 bp

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Pi 0.0281

MarkerSeek score 69.30

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 929
variable_sites: 49
pi: 0.0281
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 69.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TGGGAAGTGGAATGAGAGGT	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.0	58.0	1394-1471 bp	1.0	86.7
ndhF-rpl32_p2	TGGGAAGTGGAATGAGAGGT	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.0	58.0	1395-1472 bp	1.0	86.7
ndhF-rpl32_p3	TGGCAACTGCACCTGTAAAT	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.0	58.0	2060-2137 bp	1.0	86.1
ndhF-rpl32_p4	TGGCAACTGCACCTGTAAAT	CAGCGTGTCTACCGATTTCA	45.0	50.0	58.0	58.0	2061-2138 bp	1.0	86.1
ndhF-rpl32_p5	TGGCAACTGCACCTGTAAAT	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	58.0	57.9	2070-2147 bp	1.0	85.1