Hotspot

psbM-trnD-GUC

LSC · 29973-30701 · 729 bp

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Pi 0.00687

MarkerSeek score 64.40

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 729
variable_sites: 10
pi: 0.00687
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	TCACTTTGACTCACGGTTTT	TCAATTGGTTAGAGCACCGC	40.0	50.0	55.4	58.6	887-893 bp	1.0	57.1
psbM-trnD-GUC_p2	TCACTTTGACTCACGGTTTTT	TCAATTGGTTAGAGCACCGC	38.1	50.0	56.2	58.6	887-893 bp	1.0	55.4
psbM-trnD-GUC_p3	AATCACTTTGACTCACGGTT	TCAATTGGTTAGAGCACCGC	40.0	50.0	55.2	58.6	889-895 bp	1.0	55.2
psbM-trnD-GUC_p4	ATCACTTTGACTCACGGTTT	TCAATTGGTTAGAGCACCGC	40.0	50.0	55.2	58.6	888-894 bp	1.0	55.2
psbM-trnD-GUC_p5	AGAATGAAGAGTGCAGTAGC	TCAATTGGTTAGAGCACCGC	45.0	50.0	55.2	58.6	833-839 bp	1.0	55.1